Sequence of the novel essential gene YJU2 and two flanking reading frames located within a 3.2 kb EcoRI fragment from chromosome X of Saccharomyces cerevisiae

We have isolated and sequenced a novel gene from Saccharomyces cerevisiae coding for an essential protein of unknown function. The gene called YJU2 was borne on a chromosome X fragments shown by hybridization to intact S. cerevisiae chromosomal DNA fractionated by orthogonal pulsed field electrophoresis. Northern hybridization analysis indicated that the 0.8‐kb transcript of YJU2 is expressed in exponential‐phase cells grown in rich medium (data not shown). Figure 1 shows the nucleotide and deduced amino‐acid sequences of the 834‐bp coding region as well as the nucleotide sequences of the 5′ upstream region and of the 3′ downstream region, together with the flanking neighbouring open reading frames (ORFs), YJU1 and YJU3 .