This paper describes a recommended method for the detection and enumeration of lecithinase-positive clostridia in fecal specimens. The use of specimens treated at 80°C for 10 min in parallel with unheated specimens with both selective and nonselective media is needed for the enumeration of lecithinase-positive clostridia in fecal specimens, when the numbers are less than 104/g.
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Terada et al. (1994) studied this question.
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