Answers are grounded in this paper and cite their sources.
The ability of peptides and proteins from Bifidobacterium lactis Bb12 cytoplasm to elicit immune responses was measured in terms of murine splenocyte proliferation and cytokine secretion. Peptides and proteins from B. lactis Bb12 cytoplasm were separated into acidic and basic fractions by liquid-phase electrofocalization based on isoelectric pH. Both fractions were further fractionated by RP-HPLC based on hydrophobicity. Major peaks (of low hydrophobicity) selected in each fraction were found by mass spectrometry to contain an abundant peptide or protein with contaminants. The acidic fraction gave a stimulation index of 1.61±0.08 compared to 1.33±0.02 for the basic fraction and 2.96±0.35 for crude extract. The basic fraction stimulated IFN-γ secretion more than the acidic fraction (579±195 vs. 191±7 pg ml−1). The results demonstrate that peptides and proteins from B. lactis Bb12 exhibit immunostimulating activity, suggesting the use of cytoplasm as a nutraceutical product to enhance immune responses in children or elderly persons with reduced immune function.
No takes yet. Share an insight, caveat, or question.
Amrouche et al. (2006) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: