Zur Verwendung von 2‐Hydroxyethyl‐Methacrylat (GMA) als Einbettungsmedium bei histologischen Untersuchungen in der Phytopathologie

The use of 2‐hydroxyethyl‐methacrylate (GMA) as embedding medium for histological investigations in phytopathology A new plastic embedding technique is described for subsequent thin sectioning of plant tissues. In comparison to the paraffinmethod the GMA polymerization system is less time consuming. The excellent preservation of well‐fixed tissue is fully asserted, as the embedding medium is not removed from the sections. In lightmicroscopic studies convincing results were obtained with different staining procedures; specific evidence for polysaccharides, pectine and nucleic acids was carried out with thin sections of 2‐8 μm thickness, also by fluorescence microscopy. The GMA‐embedding technique seems to be of value for various histological investigations in phytopathology.