Serial Sectioning of Tissues in Glycol Methacrylate by Supplementary Embedding in a Paraffin-Plastic Matrix

Glycol methacrylate, while offering certain advantages over paraffin as an embedding medium, is difficult to use because it will not ribbon. Rohde (1965) developed a method for producing ribbons of methacrylate sections, but we had little success with it because the ribbons tended to fall apart when even slight stresses were applied to them. We have therefore made use of the principle of double embedding, as this has been used for obtaining serial sections of material embedded in nitrocellulose.