Key result
Children with tetralogy of Fallot or ventricular septal defect had altered thiol/disulphide homeostasis and significantly elevated serum nitric oxide levels compared to healthy controls (p<0.001).
Why the study?
Oxidative stress may contribute to congenital heart defect pathogenesis, but the role of dynamic thiol/disulphide homeostasis had not been evaluated in children with TOF and VSD.
Are there changes in thiol/disulphide homeostasis and nitric oxide levels in children with tetralogy of Fallot and ventricular septal defect compared to healthy controls?
Case-Control (n=94)
Are there changes in thiol/disulphide homeostasis and nitric oxide levels in children with tetralogy of Fallot and ventricular septal defect compared to healthy controls?
Thiol/disulphide homeostasis and nitric oxide levels are significantly altered in children with tetralogy of Fallot and ventricular septal defect, indicating a potential role of oxidative stress in these conditions.
May reflect oxidative stress in these defects; hypothesis-generating and requires prospective validation before clinical implications.
Oxidative stress may contribute to the pathogenesis of congenital heart defects, but the role of dynamic thiol/disulphide homeostasis has not been evaluated. The objective of this study was to assess whether there are changes in thiol/disulphide homeostasis and nitric oxide levels in children with tetralogy of Fallot (TOF) and ventricular septal defect (VSD). A total of 47 children with congenital heart defects (24 TOF and 23 VSD) and 47 healthy age- and sex-matched controls were included in this study. Serum total thiol and native thiol levels were measured using a novel automatic spectrophotometric method. The amount of dynamic disulphide bonds and related ratios were calculated from these values. Serum nitric oxide levels were detected using a chemiluminescence assay. We found that the average native thiol, total thiol, and disulphide levels were decreased in patients with VSD when compared with healthy individuals (p < 0.001, p < 0.001, and p < 0.01, respectively). While native thiol levels were decreased (p < 0.01), disulphide levels were elevated in the TOF group (p < 0.05). We observed marked augmentation of disulphide/native thiol (p < 0.001) and disulphide/total thiol ratios (p < 0.01) in the TOF group. However, there was a significant decrease in native thiol/total thiol ratio in patients with TOF. No significant changes in these ratios were noted in the VSD group. We detected significant elevations in serum nitric oxide levels in children with TOF and VSD (p < 0.001 for all). These results are the first to demonstrate that thiol/disulphide homeostasis and nitric oxide are associated with TOF and VSD in children.
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Temel et al. (2019) conducted a case-control in Tetralogy of Fallot and Ventricular Septal Defect (n=94). Congenital heart defects (Tetralogy of Fallot and Ventricular Septal Defect) vs. Healthy age- and sex-matched controls was evaluated on Thiol/disulphide homeostasis and serum nitric oxide levels (p=<0.001). Children with tetralogy of Fallot or ventricular septal defect had altered thiol/disulphide homeostasis and significantly elevated serum nitric oxide levels compared to healthy controls (p<0.001).
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