The photolysis of HbO2 and HbCO has been investigated with picosecond laser techniques. Transient absorption spectra were measured in the Soret and visible regions after excitation with 353- or 530-nm pulses. The photoproducts appeared within 8 psec and exhibited considerably broadened deoxyhemoglobin-like spectra, which persisted to 680 psec. The altered spectra are attributed to the production of deoxyheme conformational and spin states that might result from the intense excitation.
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Greene et al. (1978) studied this question.
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