The application of a two-dimensional paper-chromatographic technique to the analysis of phospholipids

The object of this work was to develop a satis- factory method for the quantitative analysis of yeast phospholipid mixtures that contain appreci- able amounts of lysophosphatides. The hydrolytic procedure, which has been used by Paper chromatography, on modified filter papers, has been applied to the separation of phospholipids with considerable success. In particular, the procedure developed by Horhammer and co-workers (Horhammer, Wagner & Richter, 1959; Horhammer & Richter, 1959; Horhammer, Holzl & Wagner, 1961) have also developed procedures for separating phospholipids by employing formaldehyde-treated filter paper. Though good separation of some lipids was ob- tained, the resolving power was inadequate for the chromatography of complex mixtures. The present paper describes the applicationof atwo-dimensional paper-chromatographic technique to the analysis of phospholipids. Lipid extracts from ox liver, ox brain, soya bean, Bacillus cereus and baker's yeast have been analysed to demonstrate the general application of the method.