Growth of Escherichia coli and Staphylococcus aureuson the surface of Trypticase Soy Agar (TSA) packaged with various CO 2 partial pressures (0, 20, 40, 60, 80, 100%, balance N 2 ) was compared to the control (N 2 100%) on TSA in which the pH was adjusted to equal that in CO 2 atmospheres at 15°C and 30°C. At 15°C, the biostatic effect was noted with all CO 2 partial pressures for both species. At 30°C, the biostatic effect of CO 2 was almost completely nullified for E. coli , but that for S. aureus was still effective. S. aureus was more sensitive to the inhibitory effects of CO 2 than E. coli at both the temperatures.
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Kimura et al. (1999) studied this question.
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