The maximum-slope method, previously suggested by one of the authors has proved to be more advantageously applied to the determination of the aggregation constant (Kn) and the degree of aggregation (n) in a dye solution than is Zanker’s method. Changes in the spectra of the aqueous or ethanolic solutions of Rhodamine 6G, 3,6-diaminoacridine and thionine were studied as their concentration increased by means of both the maximum-slope method and Zanker’s method. The aggregation characteristics obtained by these two methods were then compared with each other and discussed.
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Hida et al. (1970) studied this question.
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