What question did this study set out to answer?

To examine how specific chemical inhibitors of ammonia assimilation alter enzyme activity and amino acid concentrations in barley roots and shoots.

March 1, 1983PLANT PHYSIOLOGYOpen Access

Action of Inhibitors of Ammonia Assimilation on Amino Acid Metabolism in Hordeum vulgare L. (cv Golden Promise)

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To examine how specific chemical inhibitors of ammonia assimilation alter enzyme activity and amino acid concentrations in barley roots and shoots.
Grew barley (Hordeum vulgare cv Golden Promise) in a continuous culture system supplied with either 1 millimolar nitrate or 1 millimolar ammonia over a 6-hour period.
Treated plant root systems with either 1 millimolar methionine sulfoximine (MSO) or 25 micrograms per milliliter azaserine.
Measured subsequent changes in root and shoot enzyme activity, free ammonia levels, and individual amino acid pools.
Application of 1 millimolar MSO caused complete inactivation of root glutamine synthetase with negligible shoot impact, increasing root ammonia, glutamate, aspartate, serine, threonine, and asparagine while reducing glutamine.
Application of azaserine (25 micrograms per milliliter) in nitrate-grown plants induced a rapid rise in root ammonia, glutamine, and serine alongside a decline in glutamate, aspartate, and alanine, while elevating shoot glutamine.

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Implication

Experimental study reveals specific shifts in amino acid profiles following enzyme inhibition in barley, confirming the active role of the glutamine synthetase pathway.

Cite This Study

Fentem et al. (1983) studied this question.

synapsesocial.com/papers/6a844cf9d87e21f83cb299dc https://doi.org/10.1104/pp.71.3.502

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Implication

Experimental study reveals specific shifts in amino acid profiles following enzyme inhibition in barley, confirming the active role of the glutamine synthetase pathway.

Action of Inhibitors of Ammonia Assimilation on Amino Acid Metabolism in Hordeum vulgare L. (cv Golden Promise)

Key Points

Discussion

Implication

Cite This Study

Action of Inhibitors of Ammonia Assimilation on Amino Acid Metabolism in Hordeum vulgare L. (cv Golden Promise)

Key Points

Discussion

Implication

Cite This Study

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