Key result
Overexpression of IGFBP3 and demethylation treatment with 5-Aza/TSA induced apoptosis and significantly reduced cell proliferation and colony formation in HUH7 hepatocellular carcinoma cells.
Why the study?
Does IGFBP3 overexpression reduce proliferation and induce apoptosis in hepatocellular carcinoma cells?
Does IGFBP3 overexpression reduce proliferation and induce apoptosis in hepatocellular carcinoma cells?
Overexpression of IGFBP3 and its demethylation by 5-Aza/TSA reduces cell proliferation and induces apoptosis in hepatocellular carcinoma cells, suggesting a potential therapeutic target.
IGFBP3 may be a therapeutic target in HCC; leaves open translation from cell lines to patients.
BACKGROUND: The insulin-like growth factor (IGF) system comprises a group of proteins that play key roles in regulating cell growth, differentiation, and apoptosis in a variety of cellular systems. The aim of this study was to investigate the role of insulin-like growth factor binding protein 3 (IGFBP3) in hepatocellular carcinoma. MATERIALS AND METHODS: Expression of IGF2, IGFBP3, and PTEN was analyzed by qRT-PCR. Lentivirus vectors were used to overexpress IGFBP3 in hepatocellular carcinoma cell (HCC) lines. The effect of IGFBP3 on proliferation was investigated by MTT and colony formation assays. RESULTS: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells. CONCLUSIONS: Expression of IGF2, IGFBP3, and PTEN in several HCC cell lines was lower than in normal cell lines. After 5-aza-2'-deoxycytidine/ trichostatin A treatment, significant demethylation of the promoter region of IGFBP3 was observed in HCC cells. Overexpression of IGFBP3 induced apoptosis and reduced colony formation in HUH7 cells.
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Han et al. (2015) studied Hepatocellular carcinoma. IGFBP3 overexpression and 5-Aza/TSA treatment vs. Control parent lentivirus / untreated cells was evaluated on Cell proliferation and colony formation (p=<0.05). Overexpression of IGFBP3 and demethylation treatment with 5-Aza/TSA induced apoptosis and significantly reduced cell proliferation and colony formation in HUH7 hepatocellular carcinoma cells.
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