Characterization of the Humoral Immune Response Induced after Infection with Atypical Porcine Pestivirus (APPV)

Figures

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  Figure 1. Atypical porcine pestivirus (APPV) genome titers of piglets from congenital tremor (CT) affected litters (a–c) and piglets from a healthy litter (d). Genome loads of 20 piglets were investigated by real-time RT-PCR. Cq values of serum samples (y axis), the age of piglets and holding conditions (x axis) are indicated. Vertical dashed lines indicate the changes in the holding conditions. Piglets from CT affected litter 1 (a) were cross-fostered immediately after birth to a pluriparous sow with a healthy litter. Piglets from CT affected litters 2, 3 and 4 are grouped into two panels (b,c) by the occurrence of CT.

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  Figure 2. Kinetics of E2-specific antibodies induced after APPV infection. APPV E2-specific antibody levels of 20 pigs were investigated by E2 ELISA and results are given as S/P values (S/P ≤ 0.5, low; S/P = 0.5–1.0 intermediate; S/P ≥ 1.0 high reactivity). Horizontal dashed lines indicate the APPV E2-specific Ab levels as low, intermediate and high. Vertical dashed lines indicate the changes in the holding conditions. Piglets from CT affected litter 1 (a) were cross-fostered immediately after birth to a pluriparous sow with a healthy litter. (d) Piglets from CT affected litters 2, 3 and 4 are grouped into two panels (b,c) by the occurrence of CT.

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  Figure 3. Kinetics of Erns-specific antibodies induced after APPV infection. APPV Erns-specific antibody levels of 20 pigs were investigated by Erns ELISA and results are given as S/P values (S/P ≤ 0.5, low; S/P = 0.5–1.0 intermediate; S/P ≥ 1.0 high reactivity). Horizontal dashed lines indicate the APPV Erns-specific Ab levels as low, intermediate and high. Vertical dashed lines indicate the changes in the holding conditions. Piglets from CT affected litter 1 (a) were cross-fostered immediately after birth to a pluriparous sow with a healthy litter. (d) Piglets from CT affected litters 2, 3 and 4 are grouped into two panels (b,c) by the occurrence of CT.

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  Figure 4. Reactivity of the sera against the APPV E2 and Erns envelope proteins and virus neutralizing capacity. Symbols indicate the health and infection status of the piglets. Shades of the symbols indicate the age of piglets. (a) Correlation of APPV E2 antibody ELISA (y axis) and VNT results (x axis). (b) Correlation of APPV Erns antibody ELISA (y axis) and VNT results (x axis). Individual animals indicated in the figures were described in detail within the results section. P-2, P-6, P-8, P-13, P-14 and P-15 are individually presented in Figure S1.