What question did this study set out to answer?

To establish reverse phase high-pressure liquid chromatography (HPLC) as a fast and efficient separation tool for protected intermediates during the stepwise synthesis of deoxyribopolynucleotides.

April 4, 1978Biochemistry

Studies on polynucleotides. 146. High-pressure liquid chromatography in polynucleotide synthesis

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Authors

HFHans Joachim FritzMassachusetts Institute of Technology RBR BelagajeAmgen (United States)EBEugene L. BrownDartmouth College

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Implication

Laboratory study demonstrates rapid HPLC separation of protected intermediates in synthetic deoxyribopolynucleotides, highlighting a 50% reduction in total oligonucleotide synthesis time.

Key Points

To establish reverse phase high-pressure liquid chromatography (HPLC) as a fast and efficient separation tool for protected intermediates during the stepwise synthesis of deoxyribopolynucleotides.
Utilized reverse phase HPLC equipped with microparticulate octadecyl-bonded columns to resolve nucleosides, nucleotides, and protected oligonucleotides.
Assessed retention properties relative to purine and pyrimidine base identity, protecting groups, phosphate groups, and chain length.
Demonstrated preparative-scale utility during the stepwise synthesis of the model oligonucleotide d(G-G-A-A-G-C-T-T-A-A-C).
Octadecyl column retention varied systematically according to base composition, protecting group modifications, phosphate presence, and chain length.
Preparative HPLC separation enabled rapid intermediate purification, yielding up to a 50% reduction in overall oligonucleotide synthesis time.

Cite This Study

Fritz et al. (1978) studied this question.

synapsesocial.com/papers/6a8469a3c7e37ebe3168dc25 https://doi.org/10.1021/bi00600a020

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April 4, 1978Biochemistry

Studies on polynucleotides. 146. High-pressure liquid chromatography in polynucleotide synthesis

View Full Paper

Ask AI

Bookmark

Authors

HFHans Joachim FritzMassachusetts Institute of Technology RBR BelagajeAmgen (United States)EBEugene L. BrownDartmouth College

Discussion

Member takes

Implication

Laboratory study demonstrates rapid HPLC separation of protected intermediates in synthetic deoxyribopolynucleotides, highlighting a 50% reduction in total oligonucleotide synthesis time.

Key Points

To establish reverse phase high-pressure liquid chromatography (HPLC) as a fast and efficient separation tool for protected intermediates during the stepwise synthesis of deoxyribopolynucleotides.
Utilized reverse phase HPLC equipped with microparticulate octadecyl-bonded columns to resolve nucleosides, nucleotides, and protected oligonucleotides.
Assessed retention properties relative to purine and pyrimidine base identity, protecting groups, phosphate groups, and chain length.
Demonstrated preparative-scale utility during the stepwise synthesis of the model oligonucleotide d(G-G-A-A-G-C-T-T-A-A-C).
Octadecyl column retention varied systematically according to base composition, protecting group modifications, phosphate presence, and chain length.
Preparative HPLC separation enabled rapid intermediate purification, yielding up to a 50% reduction in overall oligonucleotide synthesis time.

Cite This Study

Fritz et al. (1978) studied this question.

synapsesocial.com/papers/6a8469a3c7e37ebe3168dc25 https://doi.org/10.1021/bi00600a020

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