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Captured and bound! Kdo, a monosaccharide, and an immobilized capture molecule form a covalent adduct that can be detected by adduct-specific antibodies (see scheme). The recognition yields a signal that is proportional to the amount of Kdo present in solution. This small-molecule sensor should be applicable for any small molecule that can react to give an immunogenic adduct.
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Mannerstedt et al. (2010) studied this question.
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