By exploiting the relatively slow hydration of acetaldehyde in neutral solutions of low ionic strength and the general acid-base catalysis of this hydration reaction, it is shown that the hydration of acetaldehyde does not precede its oxidation by xanthine oxidase. It follows that the aldehyde, per se, rather than its hydrate is the true substrate.
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Irwin Fridovich (1966) studied this question.
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