December 11, 1992Science
Interaction of U6 snRNA with a Sequence Required for Function of the Nematode SL RNA in Trans-Splicing
Population
Nematode spliced leader (SL) RNAs in vitro
Design
Preclinical
Authors
Discussion
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Implication
These nematode elements may guide trans-splicing studies; leaves open any conservation or role in mammalian cardiac splicing.
Key Points
- To identify the specific sequence elements within the nematode spliced leader RNA that are essential for trans-splicing activity.
- Applied chemical modification interference analysis to identify critical functional motifs within the snRNA-like domain of nematode spliced leader (SL) RNA.
- Engineered hybrid RNAs by inserting identified functional sequences into a 72-nucleotide fragment of nematode U1 snRNA to assess sufficiency in in vitro trans-splicing assays.
- Trans-splicing activity in vitro was determined to be completely independent of exon sequence or length, relying entirely on the snRNA-like domain.
- Identified two short sequence elements that are necessary and sufficient to confer trans-splicing capability to an inactive U1 snRNA fragment.
- Demonstrated that one of the critical sequence elements mediates function by base-pairing directly with U6 snRNA.
Structured PICO
P
Population
Nematode spliced leader (SL) RNAs in vitro
I
Intervention
Chemical modification interference analysis and creation of hybrid RNA (72-nucleotide fragment of nematode U1 snRNA with short sequence elements)
O
Outcome
Participation in trans-splicing reactions in vitro
Identified two short sequence elements in the snRNA-like domain of nematode SL RNA that are necessary and sufficient for trans-splicing activity, likely via base-pairing with U6 snRNA.
Cite This Study
Hannon et al. (1992) studied this question.