Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis

Figures

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  Figure 1. Output from HRV analysis program Chart (ADInstruments) under basal conditions. A – original ECG recording; B – waterfall plot of ECG; C – tachogram – RR point series; D – histogram displaying normalized RR intervals; E – spectrum – time series after resampling, power spectral density (PSD), F – Poincare Plot – each RR interval plotted against the preceding RR interval. Parameters in the time domain were determined on the original tachogram. Parameters in the frequency domain: the power spectral density was calculated by applying the fast Fourier transform (FFT) that was sampled and 1000 Hz and calculated by using 1024 points and half overlap with a Welch window. Cutoff frequencies divided the power spectrum into two main parts, LF – low frequency (0.04–1.0 Hz) and HF – high frequency (1.0–3.0 Hz)

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  Figure 2. Results of behavioral nociceptive tests and colonic propulsion test in groups: control, TNBS-treated rats, SDV – rats after subdiaphragmatic vagotomy, SDV + TNBS – vagotomized TNBStreated rats: A – mechanical allodynia threshold [g] that elicited hind paw withdrawal – PWT, B – thermal hyperalgesia threshold [s] that elicited hind paw latency – PWL, C – colonic propulsion test – expulsion time [min] *p vs. control, #p vs. SDV.

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  Figure 3. Autonomic nervous system test – results of heart rate variability (HRV) parameters in groups: control, TNBS-treated rats, SDV – rats after subdiaphragmatic vagotomy, SDV + TNBS – vagotomized TNBS-treated rats. A – heart rate (HR), B – mean RR intervals, C – total power (TP), D – low frequencies normalized (LFnu), E – high frequencies normalized (HFnu), F – LF/HF ratio *p vs. control, #p vs. SDV (see Table II).

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  Figure 4. The relation between the percentage weight gain (from day 1 to 7) and the result of histological examination (colonic wall damage score 1 to 4, as described in the methods section) in groups: control, TNBS-treated rats, SDV – rats after subdiaphragmatic vagotomy, SDV + TNBS – vagotomized TNBS-treated rats