A High Affinity Binding Site for the Polypyrimidine Tract Binding Protein (PTB) Is Located in the 5'-Untranslated Region of the Rat Proteinase α1-Inhibitor 3 Variant I Gene

As a first step towards understanding the mechanism underlying the differential gene expression of the two variants of the rat proteinase-inhibitor alpha1-inhibitor 3 (alpha1-I3) corresponding genomic clones were isolated. The 100% similarity between the sequence of one genomic clone and that of the alpha1-13 variant I cDNA strongly suggested that its 5'-sequence represented the upstream region of the corresponding gene. Several putative cis-regulatory elements were identified as well as a polypyrimidine tract located between the transcription start site of the alpha1-I3 variant I mRNA and the AUG codon. The polypyrimidine tract functions as a positive cis-element in a heterologous promoter. By electrophoretic mobility shift assays (EMSA) we have shown that a GST (glutathione S-transferase) fusion of the rat polypyrimidine tract binding protein (PTB) has a high affinity for the pyrimidine-rich sense strand but not for the complementary sequence of the 5'-untranslated region of the alpha1-I3 variant I gene.