The air pollutant ozone Is believed to exert its deleterious biological effects by the formation of free radicals. However these free radicals have not been directly measured. It has also been suggested that peroxidation of cell membrane unsaturated fatty acids is an important mechanism In ozone toxicity. Utilizing electron paramagnetic resonance technique, direct ozonization of linoleic acid produced measurable free radicals after a two-hour induction period.
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Goldstein et al. (1968) studied this question.
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