Real‐Time Scanning Force Microscopy of Macromolecular Conformational Transitions

Summary: Comb‐like macromolecules were adsorbed on mica and imaged by scanning force microscopy in real time as they underwent a transition from an extended worm‐like conformation to globuli and vice versa. The conformational transition was effected by coadsorption of ethanol and water molecules. Coadsorption of the small molecules allowed manipulation of the adherence and spreading of the macromolecules, thus effecting the reptation like stretching and collapse of the single molecules. SFM images of three individual PMA‐g‐P n BuA brush molecules on mica 27 min (left, first collapse cycle) and 18 min (right, second collapse cycle) after injection of ethanol into the sample space. image SFM images of three individual PMA‐g‐P n BuA brush molecules on mica 27 min (left, first collapse cycle) and 18 min (right, second collapse cycle) after injection of ethanol into the sample space.