The fatty acid composition of three phospholipids of boar spermatozoa from 32 ejaculates was determined; choline phosphatide, ethanolamine phosphatide, and serine phosphatide were the phospholipids in which 19 fatty acids and two aldehydes were identified. Thin-layer and gas-liquid chromatography were employed for separation of phospholipids and determination of fatty acids. Major saturated fatty acids present were palmitic (16:0), stearic (18:0), and myristic (14:0). Palmitic and stearic acid were present in highest concentrations, ranging from 8 to 19% and 4 to 36%, respectively, in the three phosphatides. Unsaturated fatty acids predominated in the choline and ethanolamine phosphatides, while saturated acids predominated in the serine phosphatides. Docosapentaenoic acid (22:5ω6) was the major unsaturated acid at a high of 38.6%. Docosahexaenoic acid (22:6) was somewhat less at 25.2%. Oleic acid (18:1ω9), linoleic acid (18:2ω6), and arachidonic acid (20:4ω6) were present in lesser quantities.
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Johnson et al. (1969) studied this question.
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