Occurrence of light saturation in the reaction of thionine (T) with Fe(2+) suggests the existence of T. Fe(2+) complexes practically indistinguishable by their absorption spectra from free thionine; photoreduction seems to occur by electron transfer in such complexes, delayed by more than 10(-5) second after light absorption. Both phenomena must be of general significance.
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Ainsworth et al. (1960) studied this question.
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