A study on the use of phenylacetyl disulfide in the solid‐phase synthesis of oligodeoxynucleoside phosphorothioates

Sulfurization of protected [ i.e. , 2‐cyanoethyl, methyl and 2‐(4‐nitrophenyl)ethyl] internucleosidic phosphite triesters can be conveniently executed with the easily accessible reagent, phenylacetyl disulfide (1). High‐quality oligodeoxynucleotides containing predetermined combinations of natural and phosphorothioate linkages can be obtained via a solid‐phase approach by using reagent 1 and 2‐(4‐nitrophenyl)ethyl‐protected phosphoramidites.