November 1, 2002Journal of Biological ChemistryOpen Access

An External Determinant in the S5-P Linker of the Pacemaker (HCN) Channel Identified by Sulfhydryl Modification

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Population

Wild-type (WT) and engineered (C303S, C318S, C347S) HCN1 channels

Comparison

External application of hydrophilic… vs Control and comparison between WT and mutant…

Design

Preclinical

Authors

TXTian XueUniversity of Science and Technology of China RLRonald A. LiUniversity of Novi Sad

Discussion

Member takes

Implication

Identifies modifiable site in HCN1; extends channel gating insights but leaves open in vivo cardiac relevance.

Structured PICO

Population

Wild-type (WT) and engineered (C303S, C318S, C347S) HCN1 channels

Intervention

External application of hydrophilic sulfhydryl-modifying agents methanethiosulfonate ethylammonium (MTSEA(+)) and methanethiosulfonate ethylsulfonate (MTSES(-))

Comparator

Control (unmodified channels) and comparison between WT and mutant channels

Outcome

Whole-cell currents, activation and deactivation kinetics, and steady-state activationsurrogate

The S5-P Cys(318) residue of the HCN1 pacemaker channel is externally accessible, demonstrating allosteric coupling between the external pore vestibule and activation gating.

Cite This Study

Xue et al. (2002) studied this question.

synapsesocial.com/papers/6a84fc6d5c0b46d4e2990de7 https://doi.org/10.1074/jbc.m204915200

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An External Determinant in the S5-P Linker of the Pacemaker (HCN) Channel Identified by Sulfhydryl Modification

Discussion

Member takes

Implication

Structured PICO

Cite This Study

An External Determinant in the S5-P Linker of the Pacemaker (HCN) Channel Identified by Sulfhydryl Modification

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider