A simple and rapid clinical routine method is described for the serial estimation of oestriol in pregnancy urine. It involves enzyme hydrolysis at an elevated temperature, ether extraction, solvent partition and colorimetric estimation by means of a modified Kober reaction. The method fulfils the established criteria of reliability, when applied to pregnancy urine specimens containing 2.0 μg oestriol per ml, or more. The procedure is so simple and rapid that the average technician can complete the analysis of 24 specimens within 5 hours.
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Torre et al. (1970) studied this question.
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