Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16

Xiao Xu; Falei Zhang; Luning Zhou; Yimin Chang; Qian Che; Tianjiao Zhu; Dehai Li; Guojian Zhang

doi:10.3390/md21040240

April 14, 2023Marine DrugsOpen Access

Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16

View Full Paper

Ask AI

Bookmark

Share

Key Points

To identify novel bioactive secondary metabolites from Streptomyces sp. XS-16 by activating silent biosynthetic pathways through the overexpression of the global regulator SCrp.
Overexpressed the native cyclic AMP receptor protein (SCrp) in Streptomyces sp. XS-16 to induce metabolite expression.
Elucidated chemical structures using nuclear magnetic resonance (NMR) spectroscopy, mass spectrometry, and electronic circular dichroism (ECD) calculations.
Assayed isolated compounds for in vitro antimicrobial and antitumor activities against tumor cell lines.
Yielded six angucycline compounds, including three previously unreported molecules (designated 1–3).
Identified potent inhibitory activity in compound 1 against multiple tumor cell lines, with IC50 values ranging from 0.32 to 5.33 μM.

Ask about this paper

Answers are grounded in this paper and cite their sources.

Implication

Experimental study reveals novel bioactive angucyclines in Streptomyces sp. XS-16 via SCrp regulator overexpression, suggesting new avenues for antitumor compound discovery.

Abstract

Six angucyclines including three unreported compounds (1–3) were isolated from Streptomyces sp. XS-16 by overexpressing the native global regulator of SCrp (cyclic AMP receptor). The structures were characterized based on nuclear magnetic resonance (NMR) and spectrometry analysis and assisted by electronic circular dichroism (ECD) calculations. All compounds were tested for their antitumor and antimicrobial activities, and compound 1 showed different inhibitory activities against various tumor cell lines with IC50 values ranging from 0.32 to 5.33 μM.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Xu et al. (2023) studied this question.

synapsesocial.com/papers/6a85151ecf83b00b9b991359 https://doi.org/10.3390/md21040240

View Full Paper

Ask AI

Bookmark

Share

Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16

Key Points

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Keep reading

Keep reading