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A simple, highly precise method has been established for the simultaneous determination of the bioactive molecules bergenin and gallic acid in three different species of Bergenia-B. ligulata (Wall) Eng., B. ciliata (Royle) Raizada, and B. stracheyi Engl. The assay combines separation and quantitative estimation of the analytes on silica gel 60GF 254 HPTLC plates with visualization under UV light and scanning at 260 nm. This study has enabled simultaneous analysis of bergenin and gallic acid in all three species.
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Srivastava et al. (2007) studied this question.
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