Rapid and quantitative screening in 96-well microplates can identify active and enantioselective hydrolases. Hydrolysis of esters releases a proton, which can be detected with pH indicators by colorimetry (figure). Using pure enantiomers, we measured the initial rates of enzyme-catalyzed hydrolysis. The relative initial rate approximates the enantioselectivity. This screening greatly speeds up selection of the best hydrolase for a synthesis.
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Janes et al. (1998) studied this question.