Methods for the determination of cortisol and corticostcrone in blood are critically reviewed with special reference to work on human blood. The period covered is 1954 to the end of 1960, but some earlier work has been included. The collection and storage of blood samples, methods of extraction, purification, separation and determination are discussed in detail. Some of the more refined methods as well as the more widely used cruder techniques are reviewed in terms of available data on their sensitivity, precision, accuracy and specificity. Data are also presented on normal plasma corticosteroid levels at various times of day.
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Braunsberg et al. (1961) studied this question.