A three-dimensional (3-D) near-infrared (NIR) Raman microprobe chemical tomography (Raman-CT) system was developed by combining a highly sensitive NIR Raman microprobe spectrometer and a two-slit confocal arrangement. The system provided a very clear 3-D NIR Raman-CT image of p−cresol (a model compound for tyrosine) in a 20-xsμm square micro-capillary cell. The image obtained using NIR laser light contained much less fluorescence interference than those obtained with visible laser light and this indicates that the system has sufficient sensitivity and spatial resolution to identify the distribution of organic or biological molecules in micro-scale samples.
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Ajito et al. (1998) studied this question.
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