Comparison of organic and inorganic germanium compounds in cellular radiosensitivity and preparation of germanium nanoparticles as a radiosensitizer

Ming-Hsing Lina, Tzu-Sheng Hsua, Pei-Ming Yanga, Meng-Yen Tsaibc, Tsong-Pyng Perngb & Lih-Yuan Lina*a Departments of Life Scienceb Materials Science and Engineering, National Tsing Hua University, Hsinchu, Taiwanc Instrument Technology Research Center, National Applied Research Laboratories, Hsinchu, Taiwan, Republic of China† Correspondence: Lih-Yuan Lin, Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, ROC, 886 3 5742693, 886 3 5715934 lylin@life.nthu.edu.twPurpose: The aim of this work is to compare the radiosensitizing effect between organic and inorganic germanium compounds and to investigate whether nanometer-sized germanium particles can act as radiosensitizers.Materials and methods: Bis (2-carboxyethylgermanium) sesquioxide (Ge-132), germanium oxide (GeO2) and germanium nanoparticles were used in this study. Cell viability was determined by clonogenic survival assay. Cellular DNA damage was evaluated by alkaline comet assay, confocal microscopy and the cellular level of phospho-histone H2AX (γ-H2AX).Results: Nanometer-sized germanium particles were fabricated. They have a similar radiosensitizing effect as that of GeO2. Conversely, Ge-132 did not enhance the radiosensitivity of cells. Comet assay was employed to evaluate the level of DNA damage and confirmed that inorganic germanium compounds enhanced cellular radiosensitivity. Notably, the comet assay indicated that the nanoparticle itself caused a higher level of DNA damage. The possibility that germanium nanoparticles per se caused DNA damage was ruled out when the cellular level of γ-H2AX was examined.Conclusions: We demonstrated that inorganic but not organic germanium compounds exerted radiosensitizing effect in cells. Nanometer-sized germanium particles were fabricated and were able to enhance the radiosensitivity of cells. Confounding effect may occur when comet assay is used to estimate the level of DNA damage in the presence of germanium nanoparticles.