A bacterial stain to demonstrate the presence of Staphylococcus aureus in harvested osteomyelitis tissue samples from a research animal model is described. A number of standard methods were studied for speed, reproducibility by relatively inexperienced personnel (workstudy students) and the final product. The improved staining technique is a combination of several protocols: Hucker Conn for crystal violet, high concentrations of iodine, 50:50 acetate:ethanol, and a modified Van Gieson counterstain. The method consistently results in high contrasting detail and good photographic quality of surrounding bony tissues in 6 μm sections of formic acid decalcified, paraffin processed bone. (The J Histotechnol 15:303, 1992)
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Skinner et al. (1992) studied this question.
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