Why the study?
Does synthetic atrial natriuretic factor induce release of vasopressin in the isolated rat posterior pituitary?
Does synthetic atrial natriuretic factor induce release of vasopressin in the isolated rat posterior pituitary?
Synthetic atrial natriuretic factor induces vasopressin release from the rat posterior pituitary, a process that appears to be mediated by specific high-affinity receptors.
ANF effects on vasopressin release in rat pituitary leave open any human relevance; no clinical implications follow from this Level 5 animal data.
The synthetic fragment (Arg 101-Tyr 126) of atrial natriuretic factor (ANF) induces release of arginine vasopressin from the isolated posterior lobe of the rat hypophysis in vitro. At a physiological concentration (3 X 10(-10)M) ANF was three times more effective than 61 mM KCL. In vitro binding studies with 125I-ANF revealed the presence of high affinity receptor sites displaying a pK = 9.9, a Kd = 0.14 nM, a Bmax = 20 fmol/posterior lobe and and IC50 = 200 pM. These results suggest that arginine vasopressin release by synthetic atrial natriuretic factor may be receptor mediated.
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Januszewicz et al. (1985) studied this question.
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