SUMMARY Amoebae of the Colonia isolate of Physarum polycephalum produce plasmodia within individual amoebal clones. This paper reports the derivation from the Colonia strain C 50 of a strain CL (Colonia Leicester) which produces plasmodia in clones with high efficiency and which completes the life cycle reliably and repeatedly in single clones. The derivation of a line CL d ( CL delayed plasmodium formation) is described and, using CL d, the construction of the isogenic heterothallic strains mt 1 ; CL and mt 2 ; CL . The above strains provide a system for the isolation and genetic analysis of mutants of P. polycephalum within a uniform genetic background, in particular mutants expressed in the plasmodium. Using this system two auxotrophic mutants have been isolated. Preliminary genetic analysis has shown that they are due to single gene mutations.
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Cooke et al. (1974) studied this question.
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