Investigation on the labile interactions of proteins and ligands using electrospray ionization mass spectrometry combined with a mathematical method

Additional supporting information may be found in the online version of this article at the publisher's web site. Figure S1. Electrospray ionization mass spectra of the mixtures of lysozyme(5 μM) with rutin (a), naringin (b) and baicalein (c) at a 1 : 8 protein/ligand molar ratio in 10 mM ammonium acetate (P represents protein, and PL represents protein–ligand complexes). Figure S2. The mass spectrum of the mixture of lysozyme (5 μM), NAG3 and rutin with the molar ratio of 1 : 1 : 40 in 10 mM ammonium acetate (P represents protein, and PL represents protein–ligand complexes). Table S1. RPLref,app/RPLref values for the mixtures of lysozyme, NAG3 and rutin. Table S2. RPLref,app/RPLref values for the mixtures of lysozyme, NAG3 and naringin. Table S3. RPLref,app/RPLref values for the mixtures of lysozyme, NAG3 and baicalein. Table S4. RPLref,app/RPLref values for the mixtures of lysozyme, NAG3 and maltose. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.