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Lipoxygenase activity in grapes (cv. Macabeo) was evidenced as an increase in absorbance at 234 nm. Lipoxygenase was isolated by ammonium sulfate fractionation, hydrophobic chromatography. Elution of the enzyme from the resin was performed by a linear gradient of (NH₄)₂SO₄ and ethylene glycol. The lipoxygenase activity was higher in partially ripe than in fully ripe grapes.
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Zamora et al. (1985) studied this question.
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