number to maintain a sufficient level of the essential gene product (see page 381)~Various plasmids have been designed to facilitate the genetic manipulation of S. cerevisiae.The most useful are the shuttle vectors which can replicate and be selected in E. coli as well as in S~cerevisiae.This permits all recombinant DNA preparative procedures to be carried out using the powerful E. coli technology.Two types of shuttle vectors are illustrated in
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Kingsman et al. (1985) studied this question.
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