Inoculating more than one branch per tree had a minimal effect on reducing experimental error, but increasing the number of trees inoculated to more than six significantly reduced the statistical variance. Inoculating six to nine trees, one branch per tree, gave high precision in the detection of differences in isolate virulence while utilizing few trees and reducing labor. Criteria for differentiating L. cincta and L. persoonii in culture were examined, and a system based on colony margin and color, size of pycnidia, and growth at 37 C was found to be suitable. Isolates of L. cincta and L. persoonii were comparable in virulence, and the geographic origin of isolates tested was not correlated with the level of virulence
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G.C. Adams (1989) studied this question.