Agromyces endophyticus sp. nov., isolated from endosphere of garlic

An aerobic, Gram-stain-positive, rod-shaped bacterial strain, designated H17E-10 T , was isolated from the endosphere of garlic ( Allium sativum ) in Jeju, Korea. Growth was observed at 10–40 °C (optimum, 30 °C) and pH 7.0–10.0 (optimum, pH 8.0), with up to 6% (w/v) NaCl (optimum, 0%). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain H17E-10 T with Agromyces soli MJ21 T (98.6%), followed by Agromyces mediolanus DSM 20152 T (98.5%) and Agromyces indicus NIO-1018 T (98.1%). According to phylogenetic analyses based on 16S rRNA gene sequences, strain H17E-10 T formed a cluster with A. soli MJ21 T , A. mediolanus DSM 20152 T and A. indicus NIO-1018 T . In contrast, the phylogenomic tree exhibited close relatedness between strain H17E-10 T and Agromyces chromiiresistens H3Y2-19a T , which supported by the highest Orthologous Average Nucleotide Identity (OrthoANI; 87.7%) and digital DNA–DNA hybridization (32.7%) values with each other. The major menaquinone (MK) and polar lipids of strain H17E-10 T were MK-13 and diphosphatidylglycerol, phosphatidylglycerol and two unidentified glycolipids, respectively, with the presence of B-type peptidoglycan. The major fatty acids (>10%) of strain H17E-10 T were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that strain H17E-10 T represents a novel species of the genus Agromyces , for which the name Agromyces endophyticus sp. nov. (type strain H17E-10 T =KACC 21836 T =NBRC 115478 T ) is proposed.