Permanent lucite 'U' microtitration plates (Flow Labora- tories Ltd.) were used. Serial 2-fold dilutions of serum in PBS containing 1 per cent. normal rabbit serum were made and an equal volume (0-025 ml.) of the sensitized erythrocyte suspension was added to the cups. The trays were carefully shaken, sealed with tape, and read after 2 hours at 20C.
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Mårdh et al. (1970) studied this question.
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