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Objectives : This study aimed to evaluate inhibitory effects of GHJ on allergic inflammatory response in human mast cells (HMC-1). Methods : To investigate the inhibitory effect of GHJ (62.5, 125, 250, 500, <TEX>1000μg/mL</TEX>), HMC-1 cells were stimulated with phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI). Enzyme-linked immunosorbent assays (ELISAs), RT-PCR and Western blot analysis were investigated using GHJ extract. Results : GHJ inhibited levels of <TEX>TNF-α</TEX> and IL-6 of <TEX>1000μg/mL</TEX> concentration in ELISA and mRNA expression. GHJ had inhibitory effects in level of MAPKs, <TEX>p-IκB-α</TEX> and p-NF-kB also. GHJ attenuated Compound 48/80-stimulated histamine release. In addition, GHJ inhibited PCA reaction in vivo. Conclusion : This study indicated that GHJ extract can inhibit allergic responses in HMC-1 cell.
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Hwang et al. (2017) studied this question.
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