Physical mapping of the FSHD gene region on chromosome 4q revealed that the physical distances between loci are smaller than expected from the genetic map.
Answers are grounded in this paper and cite their sources.
Tighter physical than genetic distances in the FSHD region cautions against cM-based assumptions; leaves open recombination rate variation for refined mapping.
We have constructed a long-range restriction map of the region on chromosome 4q that contains the gene for facioscapulohumeral muscular dystrophy (FSHD). This region contains the linkage group cen ... D4S163-D4S139-D4F35S1-D4F104S1-FSHD ... 4qter, which spans a genetic distance of about 5 cM. Pulse field gel electrophoresis (PFGE) mapping indicated that these loci span a region not more than 1 Mb. STSs were developed for several of these loci, which served to isolate four overlapping yeast artificial chromosomes (YACs). These YACs confirmed the PFGE map and have allowed us to generate a more detailed restriction map using cosmid contig mapping. The physical distances were smaller than was expected on the basis of the genetic map. Two potential HTF islands have been detected within the cloned region. One HTF island maps about 100 kb centromeric from the tandem repeats involved in the FSHD mutation, whereas the other maps within these tandem repeats.
No takes yet. Share an insight, caveat, or question.
Wijmenga et al. (1993) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: