A method is described for the bioassay of ACTH in 24-hr hypophysectomized rats. It is based on the increase in adrenal corticosterone concentration resulting from the intravenous administration of ACTH. Maximum adrenal corticosterone concentrations are attained 5 min after such an injection for the range of ACTH doses effective in this method. A rectilinear log-dose response relationship exists between the doses of 25-200 /AJ ACTH/rat. This method compares favorably in sensitivity, specificity, precision (mean -y =s/b =0.191) and ease of performance with existing methods. Resting levels of ACTH, determined in 3-5 ml samples of rat or human plasma, are readily detectable. (Endocrinology79:624,1966)
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Vernikos-Danellis et al. (1966) studied this question.