Metabolites of [³h]acetate Bound to Synaptic Vesicles Isolated From Rat Cerebral Cortex

Synaptic vesicles were isolated from rat cerebral cortex after an intraventricular injection of [ 3 H]acetate. The labelled substances bound to the synaptic vesicles were released by exposure to acid, separated from the vesicle membranes by Sephadex column chromatography and identified by thin‐layer chromatography and thin‐layer electrophoresis. The three major peaks of radioactivity were glutamate, glutamine and gamma‐aminobutyric acid. Their presence in synaptic vesicles is consistent with the concept of an integration of energy metabolism, membrane regulation and synaptic transmission.