ATP production coupled to the denitrification of nitrate in Rhizobium japonicum, grown in cultures and in bacteroids from Glycine max

The denitrification of nitrate in membrane fractions of Rhizobium japonicum CC705 grown in culture solutions with nitrate and those from bacteroids of Glycine max was coupled to ATP production. The ATP/2e − ratios for electron transfer from succinate and NADH to NO − 3 and O 2 in membrane fractions of R. japonicum grown with nitrate were: succinate to O 2 , 2.0; succinate to nitrate, 1; NADH to O 2 , 3; NADH to nitrate, 2. In membrane fractions of bacteroids the value for succinate to nitrate was also 1.