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February 19, 2001Acta Physiologica Scandinavica

Relationship between ischaemic time and ischaemia/reperfusion injury in isolated Langendorff‐perfused mouse hearts

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Why the study?

Does the duration of global ischemia affect myocardial functional recovery and creatine kinase release in isolated Langendorff-perfused mouse hearts?

Population

Isolated mouse hearts perfused in the Langendorff mode with pyruvate-containing Krebs-Hensleit (KH) buffer

Comparison

Global ischemia for 5, 15, 20, 25, 30, 45 or 60… vs Continuous perfusion (non-ischemic hearts)

Design

Preclinical

Follow-up

45 min of reperfusion

Authors

QWQing‐Dong WangASA. SwärdhPSP.‐O. Sjöquist

Discussion

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Overview

Supports model validation for ischemia/reperfusion studies in isolated hearts; leaves open translation to in vivo or clinical settings.

Structured PICO

Does the duration of global ischemia affect myocardial functional recovery and creatine kinase release in isolated Langendorff-perfused mouse hearts?

P
Population
Isolated mouse hearts perfused in the Langendorff mode with pyruvate-containing Krebs-Hensleit (KH) buffer
I
Intervention
Global ischemia for 5, 15, 20, 25, 30, 45 or 60 min followed by 45 min of reperfusion
C
Comparator
Continuous perfusion (non-ischemic hearts)
O
Outcome
Myocardial functional recovery (LV dP/dt max, LVEDP, coronary flow, heart rate) and creatine kinase (CK) releasesurrogate

The Langendorff-perfused isolated mouse heart model demonstrates an ischemia length-dependent impairment in functional recovery and increase in myocardial injury, validating its use for ischemia/reperfusion studies.

Cite This Study

Wang et al. (2001) studied this question.

synapsesocial.com/papers/6a858fe7d1e5e0588dd4e532https://doi.org/10.1046/j.1365-201x.2001.00788.x
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