This paper summarizes the authors' experience of the cultivation of promastigotes of Leishmania both for maintenance in the laboratory and for growth in mass culture. The maintenance studies were carried out with a L. donovani strain which was isolated in Murcia and cultivated in the following media: NNN, NNN with Hank's solution overlay, NNN with Brain-Heart Infusion overlay, Mathis's Medium, Weinman's Medium, Brain-Heart Infusion blood, semi-solid Brain-Heart Infusion medium, Brain-Heart Infusion/Haemin medium, Brain- Heart Infusion/lysed blood and NNN condensation liquid.For mass cultivation LV 9 (L. donovani) and LV 160 (L. mexicana amazonensis) strains from the Liverpool collection were used; these were cultivated in the following media: Brain-Heart Infusion/lysed blood, Schneider's, Homen's and Brain-Heart Infusion media.
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Marı́n et al. (1982) studied this question.
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