June 1, 1999Journal of Medical Virology

Specific detection of echoviruses 22 and 23 in cell culture supernatants by RT-PCR

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Population

20 clinical isolates of echovirus 22, 4 clinical isolates of E23, E22 and E23 prototype strains, and 64…

Design

Preclinical

Authors

MOM. Steven ObersteCenters for Disease Control and Prevention KMKaija MaherCenters for Disease Control and Prevention MPMark A. PallanschNorthwestern University

Discussion

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Implication

May enable specific E22/E23 detection in virology labs; leaves open clinical validation and diagnostic utility.

Structured PICO

Population

20 clinical isolates of echovirus 22 (E22), 4 clinical isolates of E23 (representing viruses isolated in 15 states over a 19-year period), E22 and E23 prototype strains, and 64 other enterovirus prototype strains

Intervention

RT-PCR method using E22/E23 specific primers directly in tissue culture supernatants without a viral RNA purification step

Outcome

Specific amplification and detection of E22 and E23 RNAsurrogate

A newly developed RT-PCR method allows for the specific and rapid detection of echoviruses 22 and 23 directly from tissue culture supernatants, overcoming the limitations of pan-enterovirus primers.

Cite This Study

Oberste et al. (1999) studied this question.

synapsesocial.com/papers/6a85a166ea214a590d3da240 https://doi.org/10.1002/(sici)1096-9071(199906)58:2<178::aid-jmv13>3.0.co;2-q

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Specific detection of echoviruses 22 and 23 in cell culture supernatants by RT-PCR

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Specific detection of echoviruses 22 and 23 in cell culture supernatants by RT-PCR

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider