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Crude ethanol extracts of leaves of Croton (Euphorbiaceae) species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus) were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive) fractionation and derivatisation.
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Pereira et al. (1999) studied this question.
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